High Concentration Heat-stable Alpha-Amylase HA-470

for Liquefaction of Starch Materials in Ethanol Production

DESCRIPTION

• HA-470 is a Heat-stable Alpha-Amylase produced from strains of Bacillus licheniformis through submerged fermentation and refining extraction processes. HA-470 is an endoamylase which can rapidly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.

• As a very robust Alpha-Amylase with superior thermal and pH stability, HA-470 has found its wide application in the production of ethanol.

PRODUCT SPECIFICATIONS

EFFECT OF PH

• HA-470 is active at pH from 5.2 to 6.5. For maximum activity, the optimal pH for HA-470 is 5.4 to 6.0 ( See Figure 1). The exact pH optimum depends on actual processing conditions, including temperature, substrate concentration and reaction time.

EFFECT OF TEMPERATURE

• For interval liquefaction, the temperature range of HA-470 is 80 to 100°C, the optimum temperature range is 83 to 86°C. It liquefies starch substrates promptly at 90 to 95ºC and maintains highly active at 100ºC. For consecutive liquefaction, HA-470 demonstrates exceptional thermo-stability at a temperature range of 103 to 107ºC, maintains its stability for 5 to 7 minutes and keeps on liquefying efficiently ( See Figure 2).

INHIBITORS

• Copper, titanium, and cobalt ions are moderate inhibitors for HA-470. Aluminum, lead and zinc ions act as strong inhibitors.

USAGE GUIDELINES

• In general, first adjust the pH to 5.4 to 6.0, then add HA-470. Calcium is not needed. After this enzyme is added, heat the slurry to liquefy at a temperature range of 83 to 86ºC for 90 to 150 minutes. The final DE value should be maintained at 10 to 12 ( See Figure 3 and 4).

• Exact dosage rate of HA-470 depends on the kind of starch, substrate concentration and other processing conditions. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product. A good starting point of dosage rate of HA-470 is 0.07 to 0.2kg/TDS.

PACKAGING

• HA-470 is packaged in 25kg, 30kg HDPE drums or 1125kg IBC totes. Customized packaging is available upon request.

STORAGE & SHELFLIFE

• This enzyme has been formulated for optimal stability.
• Typical shelf-life is six months provided being stored below 25°C in its original packaging, sealed and unopened, protected from the sunlight.
• Its shelf-life is eighteen months if it is stored properly at 0∼10°C. 
• Prolonged storage and/or adverse conditions such as higher temperatures may lead to higher dosage requirement, thus should be avoided.

SAFETY & ENZYME HANDLING

• Inhalation of enzyme dust and mists should be avoided.
• In case of contact with skin or eyes, promptly rinse with water for at least 15 minutes.
• For detailed handling information, please refer to the Material Safety Data Sheet.