High Efficient Enzymes For Ethanol Light To Dark Brown Liquid Alpha Amylase
|Place of Origin:||Jiangsu, China|
|Certification:||Halal, Kosher ISO9001, ISO14001, ISO22000|
|Model Number:||Heat-stable Alpha-Amylase HS-400|
Payment & Shipping Terms:
|Minimum Order Quantity:||400kg|
|Packaging Details:||25kg, 30kg plastic drums or 1125kg IBC totes|
|Delivery Time:||15-20 Days|
|Payment Terms:||T/T, L/C , D/P|
|Supply Ability:||1000MT to 1500MT Per Year|
|Grade:||Technical Grade||Form:||Liquid Form|
|Activity:||40,000 U/ml (minimum)||Appearance:||Light To Dark Brown Liquid|
Heat-stable Alpha-Amylase HS-400
Produced from Non-GMO strains of Bacillus licheniformis,
Enables better liquefaction and fermentation results
- Heat-stable Alpha-Amylase HS-400 is produced from Non-GMO strains of Bacillus licheniformis through submerged fermentation and refining extraction process.
- It is an endoamylase that can rapidly decrease the viscosity of a gelatinous starch solution by randomly hydrolyzing starch, glycogen and its degradation products within the α-D-1, 4 glucosidic bonds to produce soluble dextrins and oligosaccharides.
- HS-400 is applied to the starch liquefaction in ethanol industry.
- Declared Enzyme: Alpha-Amylase
- Systematic Name: 1,4-α-D-glucan glucanohydrolase
- Activity: 40,000 U/ml (minimum)
- Appearance: Light to dark brown liquid
- Product pH: 5.0 to 7.0
- Specific gravity: ≤1.25 g/ml
EFFECT OF PH
- HS-400 is active over a wide range of pH, depending on the application. For maximum activity, its optimal pH is from 6.0 to 6.4. At a temperature of 25°C, its stable pH range is 5.0 to 10.0. The pH optimum depends on actual process conditions including temperature, substrate concentration and processing time.
EFFECT OF TEMPERATURE
- Optimum temperature of HS-400 for interval liquefaction is above 90°C. It liquefies starch substrates efficiently at 95 to 97°C and remains highly active at 100°C. HS-400 demonstrates exceptional thermal stability at temperaturesfrom 105 to 110°C, maintains its stability for 5 to 7 minutes and keeps on liquefying efficiently. At a temperature above 105°C, the enzyme stability decreases rapidly. The exact temperature optimum depends on actual processing conditions including pH, substrate concentration and processing time.
CONCENTRATION OF CALCIUM
- The presence of calcium increases the thermal stability of HS-400. This enzyme requires a very low calcium concentration and calcium additions of 50 to 70 ppm (dry solids basis) are sufficient to ensure enzyme stability.
- Copper, titanium, and cobalt ions are moderate inhibitors for HS-400. Aluminum, lead and zinc ions act as strong inhibitors.
- In general, first adjust the pH at 6.0 to 6.4 and then add calcium (50 to 70 ppm dry solids basis) prior to adding HS-400. Calcium is not needed when extremely hard water (above 10 German degrees) is used. After the enzyme is added, heat the slurry to liquefy.
- Dosage of HS-400 depends on the actual conditions such as starch substrate, application, substrate concentration and other processing conditions. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to the routine use of this product. A good starting point for dosage rate is 0.10 to 0.25kg /TDS.
|Package:||25kg, 30kg plastic drums or 1125kg IBC totes|
|Shelf-life and Storage:||
Typical shelf-life is six months if it is stored below 25°C in its
original packaging, sealed and unopened, protected from the sunlight.
Its shelf-life will be eighteen-months if this product is being store properly at 0∼10°C.
Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement, thus should be avoided.