High Efficient Enzymes For Ethanol Light To Dark Brown Liquid Alpha Amylase

High Efficient Enzymes For Ethanol Light To Dark Brown Liquid Alpha Amylase

Product Details:

Place of Origin: Jiangsu, China
Brand Name: Boli
Certification: Halal, Kosher ISO9001, ISO14001, ISO22000
Model Number: Heat-stable Alpha-Amylase HS-400

Payment & Shipping Terms:

Minimum Order Quantity: 400kg
Price: Negotiable
Packaging Details: 25kg, 30kg plastic drums or 1125kg IBC totes
Delivery Time: 15-20 Days
Payment Terms: T/T, L/C , D/P
Supply Ability: 1000MT to 1500MT Per Year
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Detail Information

Grade: Technical Grade Form: Liquid Form
Application: For Ethanol Enzyme: Alpha-Amylase
Activity: 40,000 U/ml (minimum) Appearance: Light To Dark Brown Liquid
High Light:

fermentation enzymes


ethanol enzyme

Product Description


Heat-stable Alpha-Amylase HS-400

Produced from Non-GMO strains of Bacillus licheniformis, 

Enables better liquefaction and fermentation results




  • Heat-stable Alpha-Amylase HS-400 is produced from Non-GMO strains of Bacillus licheniformis through submerged fermentation and refining extraction process.
  • It is an endoamylase that can rapidly decrease the viscosity of a gelatinous starch solution by randomly hydrolyzing starch, glycogen and its degradation products within the α-D-1, 4 glucosidic bonds to produce soluble dextrins and oligosaccharides.
  • HS-400 is applied to the starch liquefaction in ethanol industry.




  • Declared Enzyme: Alpha-Amylase
  • Systematic Name: 1,4-α-D-glucan glucanohydrolase
  • Activity: 40,000 U/ml (minimum)
  • Appearance: Light to dark brown liquid
  • Product pH: 5.0 to 7.0
  • Specific gravity: ≤1.25 g/ml




  • HS-400 is active over a wide range of pH, depending on the application. For maximum activity, its optimal pH is from 6.0 to 6.4. At a temperature of 25°C, its stable pH range is 5.0 to 10.0. The pH optimum depends on actual process conditions including temperature, substrate concentration and processing time.



  • Optimum temperature of HS-400 for interval liquefaction is above 90°C. It liquefies starch substrates efficiently at 95 to 97°C and remains highly active at 100°C. HS-400 demonstrates exceptional thermal stability at temperaturesfrom 105 to 110°C, maintains its stability for 5 to 7 minutes and keeps on liquefying efficiently. At a temperature above 105°C, the enzyme stability decreases rapidly. The exact temperature optimum depends on actual processing conditions including pH, substrate concentration and processing time.



  • The presence of calcium increases the thermal stability of HS-400. This enzyme requires a very low calcium concentration and calcium additions of 50 to 70 ppm (dry solids basis) are sufficient to ensure enzyme stability.



  • Copper, titanium, and cobalt ions are moderate inhibitors for HS-400. Aluminum, lead and zinc ions act as strong inhibitors.



  • In general, first adjust the pH at 6.0 to 6.4 and then add calcium (50 to 70 ppm dry solids basis) prior to adding HS-400. Calcium is not needed when extremely hard water (above 10 German degrees) is used. After the enzyme is added, heat the slurry to liquefy.



  • Dosage of HS-400 depends on the actual conditions such as starch substrate, application, substrate concentration and other processing conditions. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to the routine use of this product. A good starting point for dosage rate is 0.10 to 0.25kg /TDS.



Certificate: Halal, Kosher
Package: 25kg, 30kg plastic drums or 1125kg IBC totes
Shelf-life and Storage:

Typical shelf-life is six months if it is stored below 25°C in its

original packaging, sealed and unopened, protected from the sunlight.


Its shelf-life will be eighteen-months if this product is being store properly at 0∼10°C.


Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement, thus should be avoided.


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