High Concentration Ethanol Liquefaction Enzyme  , 200000 U / ml Alpha Amylase Technical Grade

High Concentration Ethanol Liquefaction Enzyme , 200000 U / ml Alpha Amylase Technical Grade

Product Details:

Place of Origin: Jiangsu, China
Brand Name: Boli
Certification: Halal, Kosher ISO9001, ISO14001, ISO22000
Model Number: Heat-stable Alpha-Amylase HA-450

Payment & Shipping Terms:

Minimum Order Quantity: 400kg
Price: Negotiable
Packaging Details: 25kg, 30kg plastic drums or 1125kg IBC totes
Delivery Time: 15-20 Days
Payment Terms: T/T, L/C , D/P
Supply Ability: 1000MT to 1500MT Per Year
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Detail Information

Grade: Technical Grade Form: Liquid Form
Application: For Ethanol Enzyme: Alpha-Amylase
Activity: 20,000 U/ml (minimum) Appearance: Sepia Brown Liquid
High Light:

fermentation enzymes


alcohol enzyme

Product Description


Low-pH Tolerate  Heat-stable Alpha-Amylase HA-450 



EC Number :

Strains: Bacillus licheniformis

Declared Enzyme: Alpha-Amylase

Systematic Name: 1,4-α-D-glucan glucanohydrolase

Activity: 200,000 U/ml (minimum)

Appearance: Sepia brown liquid

Specific Gravity: 1.25 g/ml

Product pH: 5.0 ~ 7.0

Certificate: Halal & Kosher

Grae: Technical Grade



  • HA-450 is active over a wide pH range, depending on the application.
  • The effective pH range is 5.2~6.5.
  • For maximum activity, the optimal pH for HA-450 is 5.4 to 6.0. T
  • he exact pH optimum depends on actual processing conditions, including temperature, substrate concentration and time.



  • For interval liquefaction, the temperature range of HA-450 is 80 to 100°C, the optimum temperature range is 83 to 86°C. It liquefies starch substrates promptly at 90 to 95ºC and maintains highly active at 100ºC.
  • For consecutive liquefaction, HA-450 demonstrates exceptional thermo-stability at a temperature range of 103 to 107ºC, maintains its stability for 5 to 7 minutes and keeps on liquefying efficiently.



  • The presence of calcium increases the thermal stability of HA-450. This enzyme requires a very low calcium concentration and calcium additions of 20 to 50 ppm (dry solids basis) are sufficient to ensure enzyme stability.




  • Copper, titanium, and cobalt ions are moderate inhibitors for HA-450. Aluminum, lead and zinc ions act as strong inhibitors.



  • In general, first adjust the pH to 5.4 to 6.0, then add HA-450. Calcium is not needed. After this enzyme is added, heat the slurry to liquefy at a temperature range of 83 to 86ºC for 90 to 150 minutes. The final DE value should be maintained at 10 to 12.
  • Dosage of HA-450 is dependent on the starch substrate, application, process, substrate concentration and other factors.
  • To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product.
  • A good starting point for dosage rate is 0.1 to 0.2kg/TDS.



  • HA-450 is packaged in 25kg or 30kg non-hazardous plastic drums, or 1125kg IBC totes. Customized packaging is available upon request.
  • This enzyme has been formulated for optimal stability. Typical shelf-life is six months provided being stored below 25°C in its original packaging, sealed and unopened, protected from the sunlight.
  • Its shelf-life will be eighteen months if it is stored properly at 0~10°C.
  • Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement.



  • Inhalation of enzyme dust and mists should be avoided.
  • In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes.
  • For detailed handling information, please refer to the appropriate Material Safety Data Sheet.


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