40000 U / ml Enzymes For Ethanol Stable Activity Heat Stable Alpha Amylase HA - 400 Low pH

40000 U / ml Enzymes For Ethanol Stable Activity Heat Stable Alpha Amylase HA - 400 Low pH

Product Details:

Place of Origin: Jiangsu, China
Brand Name: Boli
Certification: Halal, Kosher ISO9001, ISO14001, ISO22000
Model Number: Heat-stable Alpha-Amylase HA-400

Payment & Shipping Terms:

Minimum Order Quantity: 400kg
Price: Negotiable
Packaging Details: 25kg, 30kg plastic drums or 1125kg IBC totes
Delivery Time: 15-20 Days
Payment Terms: T/T, L/C , D/P
Supply Ability: 1000MT to 1500MT Per Year
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Detail Information

Application: For Ethanol Enzyme: Alpha-Amylase
Activity: 40,000 U/ml (minimum) Appearance: Sepia Brown Liquid
Strain: Bacillus Licheniformis Specific Gravity:: ≤1.25 G/ml
High Light:

fermentation enzymes


ethanol enzyme

Product Description


Low-pH Tolerate Heat-stable Alpha-Amylase HA-400 

Halal & Kosher Certified




  •  Alpha-Amylase HA-400 is derived from strains of Bacillus licheniformis using submerged fermentation and refining extraction techniques.
  • HA-400 is an endoamylase which can rapidly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.



Declared Enzyme Alpha-Amylase
Systematic Name 1,4-α-D-glucan glucanohydrolase
Activity 40,000 U/ml (minimum)
Appearance Sepia brown liquid
Product pH 5.0 ~ 7.0
Specific gravity 1.25 g/ml



40000 U / ml Enzymes For Ethanol Stable Activity Heat Stable Alpha Amylase HA - 400 Low pH 0


  • The effective pH range for HA-400 is from 5.2 to 6.5 with its optimal pH range from 5.6 to 6.2. The exact pH optimum depends on actual processing conditions, including temperature, substrate concentration and time.



  • For interval liquefaction, the temperature range of HA-400 is 80 to 90°C, the optimum temperature range is 83 to 88°C. It liquefies starch substrates promptly at 90 to 95ºC and maintains highly active at 100ºC. For consecutive liquefaction, HA-400 demonstrates exceptional thermo-stability at a temperature range of 103 to 107ºC, maintains its stability for 5 to 7 minutes and keeps on liquefying efficiently.



  • Copper, titanium, and cobalt ions are moderate inhibitors for HA-400. Aluminum, lead and zinc ions act as strong inhibitors.



  • In general, first adjust the pH to 5.6 to 6.2, then add HA-400. After this enzyme is added, heat the slurry to liquefy at a temperature range of 83 to 88ºC for 90 to 150 minutes. The final DE value should be maintained at 10 to 12.
  • Dosage of HA-400 is dependent on the actual processing conditions such as kinds of starch, substrate concentration, process temperature, pH and reaction time, etc.
  • To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product.
  • A good starting point for dosage rate is 0.4 to 0.8 kg/TDS.



Package: 25kg, 30kg plastic drums or 1125kg IBC totes
Shelf-life and Storage:

Typical shelf-life is six months if it is stored below 25°C in its

original packaging, sealed and unopened, protected from the sunlight.


Its shelf-life will be eighteen months if this product is stored properly at 0∼10°C.


Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement, thus should be avoided.




  • Inhalation of enzyme dust and mists should be avoided. 
  • In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Material Safety Data Sheet.

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