High Concentration Liquefaction Enzyme HT-800

High Concentration Liquefaction Enzyme HT-800

Product Details:

Place of Origin: Jiangsu China
Brand Name: Boli
Certification: Halal, Kosher ISO9001, ISO14001, ISO22000
Model Number: Heat-stable Alpha-Amylase HT-800

Payment & Shipping Terms:

Minimum Order Quantity: 400kg
Price: Negotiable
Packaging Details: 25kg, 30kg HDPE drums, 1125 IBC totes for liquid
Delivery Time: 15-20 days
Payment Terms: T/T, L/C , D/P
Supply Ability: 1000MT to 1500MT Per Year
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Detail Information

Grade: Food Grade Form: Liquid Form
Application: Heat-stable Alpha-Amylase For Starch Sugar Concentration: High Concentration
Appearance: Light To Dark Brown Liquid Activity: 80,000 U/ml (Minimum)
High Light:

powdered enzyme amylase


fungal alpha amylase


Liquefaction Enzyme HT 800

Product Description


High Concentration Liquefaction Enzyme Heat - Stable Alpha Amylase HT-800 for Food Use

Heat-stable Alpha-Amylase HT-800



Food Grade Heat-stable Alpha-Amylase Low pH Tolerate For Starch Sugar



Halal & Kosher Certified




  • HT-800 is a low pH tolerated Heat-stable Alpha-Amylase ( EC 1,4-α-D-glucan glucanohydrolase) produced from selected strains of Bacillus licheniformis through submerged fermentation and refining extraction processes.
  • As an endoamylase, HT-800 can quickly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.


HT-800 is a robust Heat-stable Alpha-Amylase tolerates to low pH conditions specially designed for the production of starch sugar.


High Concentration Liquefaction Enzyme HT-800 0





 Declared Enzyme  Alpha-Amylase
 Systematic Name  1,4-α-D-glucan glucanohydrolase
EC Number EC
 Activity  80,000 U/ml (Minimum)
 Appearance  Sepia brown 
Physical Form Liquid
 Product pH  5.0 to 7.0
 Specific gravity  ≤1.25 g/ml


High Concentration Liquefaction Enzyme HT-800 1





  HT-800 is can work at pH from 5.0 to 7.0.


  For maximum activity, the optimal pH range for HT-800 is 5.4 to 6.0.


  The exact pH optimum depends on the actual  processing conditions,

  including temperature,  substrate concentration and reaction time, etc.


  For interval liquefaction, the optimum temperature for  HT-800 is

  above 90°C. It liquefies starch substrates  promptly at 95 to 97°C and

  maintains highly active at 100°C.


  For consecutive liquefaction, HT-800 demonstrates an  exceptional

  thermal stability from 105 to 110°C, maintains its stability for 5 to 7

  minutes and keeps on  liquefying efficiently.






  • The presence of calcium increases the thermal stability of HT-800.
  • This enzyme requires a very low calcium concentration of 20 to 50 ppm (dry solids basis) to ensure its enzyme stability.



  • Copper, titanium, and cobalt ions are moderate inhibitors for HT-800.
  • Aluminum, lead and zinc ions act as strong inhibitors for HT-800..



  • For industrial application of one-time spray liquefaction, first prepare 30∼35% w/w starch slurry, adjust pH 5.4∼6.0, then add this product at a dosage rate of 0.20∼0.30 kg/TDS.
  • Liquefy at 105∼107°C for 5 to 7 minutes, flash cooling down to around 97°C, keep on liquefying at temperature above 95°C for 90 to 120 minutes so as to reach required DE value.
  • In case the starch content is above 35%, recommended dosage rate is 0.30∼0.4kg/TDS.
  • Exact dosage of HT-800 depends on the actual processing conditions such as starch substrate, liquefaction equipment, pH, temperature, processing time, etc.
  • To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product.




  • 25kg or 30kg HDPE plastic drums
  • 1125kg IBC totes
  • Cutomized packaging available upon request

 Shelf-life and Storage:

  • Typical shelf-life is six months if it is stored below 25°C in its  original packaging, sealed and unopened, protected from sunlight.
  • Its shelf-life will be eighteen-months if this product is being store properly at 0∼10°C.
  • Prolonged storage and/or adverse conditions such as higher temperatures  may lead to higher dosage requirement, thus should be avoided.


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