High Purity Technical Grade Ethanol Enzyme , 400000 U/ml Liquefaction Enzyme

High Purity Technical Grade Ethanol Enzyme , 400000 U/ml Liquefaction Enzyme

Product Details:

Place of Origin: Jiangsu, China
Brand Name: Boli
Certification: Halal, Kosher ISO9001, ISO14001, ISO22000
Model Number: Heat-stable Alpha-Amylase HA-580

Payment & Shipping Terms:

Minimum Order Quantity: 400kg
Price: Negotiable
Packaging Details: 25kg, 30kg plastic drums or 1125kg IBC totes
Delivery Time: 15-20 Days
Payment Terms: T/T, L/C , D/P
Supply Ability: 1000MT to 1500MT Per Year
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Detail Information

Grade: Technical Grade Application: Ethanol Production
Enzyme: Alpha-Amylase Activity: 400,000 U/ml (minimum)
Appearance: Sepia Brown Liquid Strains: Bacillus Licheniformis
High Light:

alcohol enzyme


ethanol enzyme

Product Description


Heat-stable Alpha-Amylase HA-580


High Concentration Low pH Tolerate Liquefaction Enzyme for Ethanol Production

Strains: Bacillus licheniformis
Activity: 400,000 U/ml (minimum)
Certificate: Halal, Kosher
Grade Technical Grade
EC Number:
Declared Enzyme: Alpha-Amylase
Systematic Name: 1,4-α-D-glucan glucanohydrolase
Appearance: Light to dark brown liquid
Product pH: 5.0 to 7.0
Specific gravity: 1.25 g/ml


HA-580 is a heat-stable alpha-amylase derived from robust strains of Bacillus licheniformis through

submerged fermentation and refining extraction techniques. HA-580 is an endoamylase which can rapidly decrease

viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products

within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.
With robust activity and low pH stability, HA-580 is specially designed for 

liquefaction of starch materials in ethanol production.



  • HA-580 is active over a wide pH range from 5.2 to 6.5 with its optimal pH range from 5.4 to 6.0. The exact pH optimum depends on actual processing conditions, including temperature, substrate concentration and reaction time.



  • For interval liquefaction, the temperature range of HA-580 is 80 to 100°C, the optimum temperature range is 83 to 86°C. It liquefies starch substrates promptly at 90 to 95ºC and maintains highly active at 100ºC. For consecutive liquefaction, HA-580 demonstrates exceptional thermo-stability at temperatures from 103 to 107ºC, keeps on liquefying efficiently for 5 to 7 minutes.



  • Copper, titanium, and cobalt ions are moderate inhibitors for HA-580. Aluminum, lead and zinc ions act as strong inhibitors.



  • In general, first adjust the pH to 5.7 to 5.9, then add HA-580. Calcium is not needed. After this enzyme is added, heat the slurry to liquefy at a temperature range of 83 to 86ºC for 90 to 150 minutes. The final DE value should be maintained at 10 to 12.
  • Dosage of HA-580 is dependent on the starch substrate, application, process, substrate concentration and other factors. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product. A good starting point for dosage rate is 0.05 to 0.1kg/TDS.



  • HA-580 is packaged in 25kg,30kg HDPE plastic drums or 1125kg IBC totes. Customized packaging is available upon request.
  • This enzyme has been formulated for optimal stability. Typical shelf-life is six months provided being stored below 25°C in its original packaging, sealed and unopened, protected from the sunlight. Its shelf-life will be eighteen months if it is stored properly at 0~10°C. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement.


High Purity Technical Grade Ethanol Enzyme , 400000 U/ml Liquefaction Enzyme 0




  • Inhalation of enzyme dust and mists should be avoided. 
  • In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes.
  • For detailed handling information, please refer to the appropriate Material Safety Data Sheet.

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