Product Description

High Efficient Alpha Amylase Enzyme Alpha Starch Enzyme Liquefaction Enzyme

Heat-stable Alpha-Amylase HTA-430

Low pH Tolerant High Temperature Liquefaction Enzyme For Starch Sugar

Halal & Kosher Certified

EC 3.2.1.1

1,4-α-D-glucan glucanohydrolase

Activity ≥ 120,000 U/ml  

Appearance: Sepia brown Liquid

Specific Gravity: 1.10 to 1.25 g/ml

Product pH: 5.5 ~ 7.0

PRODUCT DESCRIPTION

• HTA-430 is a low pH tolerated Heat-stable Alpha-Amylase produced from selected strains of Bacillus licheniformis through submerged fermentation and refining extraction processes.

• It is a liquid endoamylase that can rapidly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.

• As a robust Alpha-Amylase with superior thermal and low pH stability, HTA-430 has found its wide application in the production of starch sugar.

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EFFECT OF TEMPERATURE

• For interval liquefaction process, the optimum temperature of HTA-430 is above 90°C. It liquefies starch substrates promptly at 95 to 97°C and maintains highly active at 100°C.
• For consecutive liquefaction process, HTA-430 demonstrates exceptional thermal stability at temperatures from 105 to 110°C and keeps on liquefying efficiently for 5 to 7 minutes.

EFFECT OF PH

• HTA-430 can work at pH ranging from 5.0 to 6.2 with its optimal pH range from 5.4 to 6.0.
• The exact pH optimum depends on the actual processing conditions, including temperature, substrate concentration and reaction time.

BENEFIT FOR STARCH SUGAR INDUSTRY

Use of HTA-430 can benefit starch sugar industry in the following aspects:

• Superior stability with high temperature and low pH
• Robust enzyme activity ensures a lower dosage rate
• Effective liquefaction of various starch materials, such as tapioca, corn, wheat, rice, etc.
• Decrease viscosity of starch quickly, capable to liquefy substrates of high solid contents
• Eliminate undesirable color substances and non-fermentable sugars
• Superior performance without adding calcium ion under majority processing conditions

USAGE GUIDELINES

• For industrial application of one-time spray liquefaction, first prepare 30 to 35% w/w starch slurry; no need to adjust pH in general, but when the pH is lower than 5.4, it has to be adjusted to the range of 5.5 to 5.9. Then add this product at a dosage rate of 0.18 to 0.25 kg/TDS. Liquefy at 105 to 107°C for 5 to 7 minutes, flash cooling down to around 95°C, keep on liquefying at temperature above 95°C for 90 to 120 minutes so as to reach required DE value.

• Exact dosage rate of HTA-430 depends on the actual processing conditions such as starch substrate, liquefaction equipment, pH, temperature, processing time, etc. To optimize its dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product.

CONCENTRATION OF CALCIUM

• The presence of calcium increases the thermal stability of HTA-430. This enzyme requires a very low calcium concentration of 5 to 20 ppm to ensure its enzyme stability.

SAFE HANDLING OF ENZYMES

• Enzyme is protein. Inhalation of enzyme dust and mists should be avoided. In case of contact with skins or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Safety Data Sheet.