BOLI Liquefaction Enzyme Heat Stable Alpha Amylase HT-800 For Starch Sugar Fermentation

BOLI Liquefaction Enzyme Heat Stable Alpha Amylase HT-800 For Starch Sugar Fermentation

Product Details:

Place of Origin: Jiangsu China
Brand Name: Boli
Certification: Halal, Kosher ISO9001, ISO14001, ISO22000
Model Number: Heat-stable Alpha-Amylase HT-800

Payment & Shipping Terms:

Minimum Order Quantity: 400kg
Price: Negotiable
Packaging Details: 25kg, 30kg HDPE drums, 1125 IBC totes for liquid
Delivery Time: 15-20 days
Payment Terms: T/T, L/C , D/P
Supply Ability: 1000MT to 1500MT Per Year
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Detail Information

Grade: Food Grade Application: Heat-stable Alpha-Amylase For Starch Sugar
Appearance: Light To Dark Brown Liquid Activity: 80,000 U/ml (Minimum)
Systematic Name:: EC, 1,4-α-D-glucan Glucanohydrolase Specific Gravity:: 1.10 To 1.25 G/ml
Free Sample: Available
High Light:

powdered enzyme amylase


fungal alpha amylase

Product Description


Heat-stable Alpha-Amylase HT-800



Food Grade Heat-stable Alpha-Amylase


Rapidly Decrease the Viscosity of Gelatinous Starch solutions



Halal & Kosher Certified





HT-800 is a low pH compatible heat-stable alpha-amylase produced from selected strains of Bacillus licheniformis through submerged fermentation and refining extraction processes. It is an endoamylase which can rapidly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.

With its robust alpha-amylase activity and superior thermal stability, HT-800 has found its wide application in the production of starch sugar.


HT-800 is a robust Heat-stable Alpha-Amylase tolerates to low pH conditions specially designed for the production of starch sugar.


BOLI Liquefaction Enzyme Heat Stable Alpha Amylase HT-800 For Starch Sugar Fermentation 0




  1.  Superior stability with low pH
  2.  Decrease viscosity of starch quickly
  3.  Eliminate undesirable color substances and non-fermentable sugars
  4.  Effective liquefaction of various starch materials, such as tapioca, corn, wheat, rice, etc.
  5.  Superior performance without adding calcium ion under majority processing conditions





 Declared Enzyme  Alpha-Amylase
 Systematic Name  1,4-α-D-glucan glucanohydrolase
EC Number EC
 Activity ≥ 80,000 U/ml
 Appearance  Sepia brown Liquid
 Product pH  5.0 to 7.0
 Specific gravity  1.10~1.25 g/ml


BOLI Liquefaction Enzyme Heat Stable Alpha Amylase HT-800 For Starch Sugar Fermentation 1





HT-800 can work at pH ranging from 5.0 to 7.0 with its optimal pH range from 5.4 to 6.0.

The exact pH optimum depends on the actual processing conditions, including temperature, substrate concentration and reaction time.


For interval liquefaction process, the optimum temperature of HT-800 is above 90°C. It liquefies starch substrates promptly at 95 to 97°C and maintains highly active at 100°C.


For consecutive liquefaction process, HT-800 demonstrates exceptional thermal stability at temperatures from 105 to 110°C and keeps on liquefying efficiently for 5 to 7 minutes.





  • The presence of calcium increases the thermal stability of HT-800.
  • This enzyme requires a very low calcium concentration of 20 to 50 ppm (dry solids basis) to ensure its enzyme stability.



  • Copper, titanium, and cobalt ions are moderate inhibitors for HT-800.
  • Aluminum, lead and zinc ions act as strong inhibitors for HT-800.


  • For industrial application of one-time spray liquefaction, first prepare 30∼35% w/w starch slurry, adjust pH 5.4∼6.0, then add this product at a dosage rate of 0.20∼0.30 kg/TDS.
  • Liquefy at 105∼107°C for 5 to 7 minutes, flash cooling down to around 97°C, keep on liquefying at temperature above 95°C for 90 to 120 minutes so as to reach required DE value.
  • In case the starch content is above 35%, recommended dosage rate is 0.30∼0.4kg/TDS.
  • Exact dosage of HT-800 depends on the actual processing conditions such as starch substrate, liquefaction equipment, pH, temperature, processing time, etc.
  • To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product.




  • 25kg or 30kg HDPE plastic drums
  • 1125kg IBC totes
  • Cutomized packaging available upon request

 Shelf-life and Storage:

  • Typical shelf-life is six months if it is stored below 25°C in its  original packaging, sealed and unopened, protected from sunlight.
  • Its shelf-life will be eighteen-months if this product is being store properly at 0∼10°C.
  • Prolonged storage and/or adverse conditions such as higher temperatures  may lead to higher dosage requirement, thus should be avoided.


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