• Heat Stable Amylase Enzyme NON - GMO , Fungal Alpha Amylase High Temperature Resistant
Heat Stable Amylase Enzyme NON - GMO , Fungal Alpha Amylase High Temperature Resistant

Heat Stable Amylase Enzyme NON - GMO , Fungal Alpha Amylase High Temperature Resistant

Product Details:

Place of Origin: Jiangsu China
Brand Name: Boli
Certification: Halal, Kosher ISO9001, ISO14001, ISO22000
Model Number: Heat-stable Alpha-Amylase HS-320

Payment & Shipping Terms:

Minimum Order Quantity: 400kg
Price: Negotiable
Packaging Details: 25kg, 30kg HDPE drums, 1125 IBC totes for liquid
Delivery Time: 15-20 days
Payment Terms: T/T, L/C , D/P
Supply Ability: 1000MT to 1500MT Per Year
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Detail Information

Application: Heat-stable Alpha-Amylase For Starch Sugar Certificate: Both Halal And Kosher Certified
Appearance: Sepia Brown Liquid Activity: 40,000 U/ml (Minimum)
Grade: Food Grade Form: Liquid Form
High Light:

powdered enzyme amylase


alpha and beta amylase

Product Description

Heat-stable Alpha-Amylase HS-320



Food Grade NON-GMO High Temperature Liquefaction Amylase 



Reduce viscosity quickly, 

Eliminate non-fermentable sugars,

Decrease  formation of chromophore,

Robust Alpha-Amylase activity capable to liquefy

Starch materials of tapioca/cassava, corn, wheat,rice, etc.





  • HS-320 is Heat stable Alpha-Amylase produced from  NON-GMO strains of Bacillus licheniformis through submerged fermentation and refining extraction process. 
  • HS-320 is an endoamylase, which rapidly decreases the viscosity of a gelatinous starch solution by randomly hydrolyzing starch, glycogen and its degradation products within the α-D-1, 4 glucosidic bonds to produce soluble dextrins and oligosaccharides.



EC Number: EC
Systemic Name:1,4-α-D-glucan glucanohydrolase

Alpha-Amylase Activity:  ≥40,000 U/ml

Appearance: Sepia brown liquid

Specific gravity: ≤ 1.25 g/ml

Product pH: 5.0 ~ 7.0



  • For industrial application of one-time spray liquefaction, first prepare 30∼35% w/w starch slurry, adjust pH 6.0 ∼ 6.2, add calcium ion 50∼70 mg/L, then add HS-320 at a dosage rate of 0.4 ∼0.6 kg/TDS. Liquefy at 105∼110°C for 5 to 7 minutes, flash cooling down to around 97°C, keep on liquefying at a temperature above 95°C for 90∼ 120 minutes so as to reach required DE value. If calcium ion concentration in tap water is already sufficient, it is no need to add calcium.
  • The actual dosage rate of HS-320 depends on the actual situation including application fields, starches, substrate concentration, temperatures, pH and other processing conditions. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to the routine use of HS-320.



  • Optimum temperature of HS-320 for interval liquefaction is above 90°C.
  • HS-320 liquefies starch substrates efficiently at 95 to 97°C and is still highly active at 100°C.
  • This product demonstrates exceptional thermal stability at temperatures from 105 to 110°C, maintains its stability for 5 to 7 minutes and keeps on liquefying efficiently.
  • At a temperature above 105°C, the enzyme stability decreases rapidly.
  • The exact temperature optimum depends on actual processing conditions including pH, substrate concentration and processing time.



  • HS-320 is active over a wide pH range, depending on the application.
  • For maximum activity, the optimal pH is 6.0 to 6.4.
  • The stable pH range is 5.0 to 10.0 at 25°C.
  • The exact pH optimum depends on actual process conditions, including temperature, substrate concentration and processing time, etc.



  • Copper, titanium, and cobalt ions are moderate inhibitors for HS-320
  • Aluminum, lead and zinc ions act as strong inhibitors



  • 25kg, 30kg plastic drums or 1125kg IBC totes
Shelf-life &Storage:
  • Typical shelf-life is six months if it is stored below 25°C in itsoriginal packaging, sealed and unopened, protected from the sunlight.
  • Its shelf-life will be eighteen-months if this product is being store properly at 0∼10°C.
  • Prolonged storage and/or adverse conditions such as higher temperaturesmay lead to a higher dosage requirement, thus should be avoided.


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